Journal article
Supramolecular Assembly of Polyphenols and Nucleic Acids by Thermal Cycling for Immune Cell Activation
Yijiao Qu, Haiyan Zhu, Zhixing Lin, Domitilla Vanni, Sukhvir Kaur Bhangu, Brendan Dyett, Marc-Antoine Sani, Christina Cortez-Jugo, Frank Caruso, Francesca Cavalieri
Chemistry of Materials | American Chemical Society (ACS) | Published : 2023
Abstract
Supramolecular assembly of polyphenols and biomacromolecules (proteins and nucleic acids) has emerged as a versatile and simple strategy to construct nanomaterials with biological activity. Here, we report a strategy to finely control the supramolecular assembly of tannic acid and oligonucleotides into uniform and stable nanoparticles by exploiting the thermal cycling of tannic acid. The equilibrium of complexation is investigated, and individual nanoparticles are resolved with nanoscale resolution by using stochastic optical reconstruction microscopy. The nanoparticles incorporating cytosine phosphoguanine (CpG) oligonucleotides are efficiently taken up by cells and trafficked via endo/lyso..
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Grants
Awarded by University of Melbourne
Funding Acknowledgements
F. Cavalieri acknowledges the award of an RMIT Vice Chancellor Senior Research Fellowship, and F. Caruso acknowledges the award of a National Health and Medical Research Council Leadership Fellowship (GNT2016732). Y.Q. acknowledges support from the China Scholarship Council. This project received funding from the European Union Horizon 2020 Research and Innovation Program under the H2020 Marie Sklodowska-Curie Actions Grant Agreement No. 872233 ("PEPSA-MATE"). This work was supported by the Australian Research Council under a Discovery Project scheme (F. Cavalieri, C.C.-J., M.S., DP210101792). This work was performed in part at the Materials Characterization and Fabrication Platform (MCFP) at The University of Melbourne and the Victorian Node of the Australian National Fabrication Facility (ANFF).